Extended Data Fig. 1: ORM2 deficiency exacerbates TG accumulation.
From: Orosomucoid 2 maintains hepatic lipid homeostasis through suppression of de novo lipogenesis
Related to Figs. 2 and 3. a-i, 8-week-old ORM2 wild-type (WT), heterozygous (HE) and knockout (KO) male mice were fed a high-fat-diet for 8 weeks. (a-c) Plasma LDL-cholesterol (a), ALT (b), AST (c) levels. WT: n = 4 biologically independent mice. HE and KO: n = 6 biologically independent mice per condition. d, Relative mRNA levels of genes related to hepatic inflammation in the liver. n = 4 biologically independent mice per condition. (e-h) Body weight (e), plasma leptin levels (f), iWAT weight (g) and BAT weight (h) of three groups of mice. WT: n = 4 biologically independent mice. HE and KO: n = 6 biologically independent mice per condition. i, H&E staining of WAT and BAT. Scale bar, 50 μm. Representative result from three biologically independent mice. j-n, 8-week-old ORM2 WT, HE and KO female mice were fed a high-fat-diet for 8 weeks. Body weight (j), liver/body weight ratio (k), hepatic (l) and plasma TG (m) levels. WT and HE: n = 6 biologically independent mice per condition. KO: n = 4 biologically independent mice. n, H&E and Oil Red O staining of liver sections. Scale bar, 50 μm. Representative result from three biologically independent mice. Data are shown as mean ± SEM. (a-h, j-m). P values are determined by one-way ANOVA followed by Dunnett’s multiple comparisons test (a-d, f-h, j-m) and two-way ANOVA followed by Dunnett’s multiple comparisons test (e).
