Fig. 3: HDAC mediates Nnat+/-p-driven β-cell hyperplasia.
From: Independent phenotypic plasticity axes define distinct obesity sub-types
a, Heat map showing the expression of 552 differentially expressed genes (DEGs), defined as Nnat+/-p-Heavy and Nnat+/-p-Light islets transcriptome, in the indicated animals. b, Volcano plot showing DEGs and highlighting the enriched or depleted biologically relevant genes in Nnat+/-p-Heavy morphs. P values as assessed by negative binomial generalized linear model. c, The cytoscape plot of the GSEA showed the enriched or depleted gene sets in Nnat+/-p-Heavy morphs. TFs, transcription factors. d, PCA demonstrated transcriptional similarity of Nnat+/-p-Heavy-like at early stages (3 weeks) and Nnat+/-p-Heavy morphs at late stages (6 weeks) apart from Nnat+/-p-Light-like at early stages (3 weeks) and Nnat+/-p-Light morphs at late stages (6 weeks). e, Heat map shows HDAC gene set leading-edge gene expression in Nnat+/-p-Heavy-like and Nnat+/-p-Light-like morphs (early stage) and Nnat+/-p-Heavy and Nnat+/-p-Light morphs (late stage). f–h, Proliferating β-cells were counted by EdU-incorporation from Nnat+/-p-Heavy and Nnat+/-p-Light morphs and are normalized to WT littermates in untreated (control, f), HDACi-treated (g) and HATi-treated (h) conditions. At least 19 islets were quantified and plotted per condition.
