Extended Data Fig. 1: Paternal but not maternal Nnat deletion causes bi-stable overgrowth.
From: Independent phenotypic plasticity axes define distinct obesity sub-types
a, The body composition was shown for 16 weeks old of F1 female wild-type and Nnat+/-p animals from Nnat+/-p x FVBN/J crosses. b-c, The body composition was shown for 16 weeks old F1 male (b) and female (c) wild-type and Nnatm/+ animals from Nnat-m/+ x FVBN/J crosses. d-e, The Nnat+/-p male (d) and female (e) body composition was plotted for the Nnat+/-p colony in the vivarium, VAI, U.S.A. f, Genotyping (DNA) was confirmed in the indicated Nnat+/-p morphs and WT littermate. Nnat mRNA expression was confirmed in metabolic tissues (adipocytes, islet, pituitary gland and hypothalamus) from male Nnat+/-p mice. Nnat mRNA expression was measured from two independent sets of littermate matched animals. The experiments were repeated independently 3 times with similar results. g, Body composition was measured by EchoMRI on 16-week-old wild-type (WT), Trim28D9/+, Nnat+/p and Nnat+/p Trim28D9/+ male progeny from F1 of B6.Nnat+/-p x FVB.Trim28D9/+ crosses. Contour plots highlight main clusters identified by Gaussian finite mixture modeling. h, Trim28 and Nnat (as control) gene expression were shown from islet (left) and adipocyte (right) transcriptomes from Nnat+/-p colony. Each group had at least 3 animals. All data were plotted as mean ± SEM, ns (not significant), **** (adjusted p < 0.0001) by one-sided Tukey’s multiple comparisons test.
