Extended Data Fig. 5: Effects of various antagonists on SETEM and motor coordination in mice, Related to Fig. 3. | Nature Metabolism

Extended Data Fig. 5: Effects of various antagonists on SETEM and motor coordination in mice, Related to Fig. 3.

From: Combined alcohol and cannabinoid exposure leads to synergistic toxicity by affecting cerebellar Purkinje cells

Extended Data Fig. 5

a,b, Effects of AM251 (i.p., 5.0 mg/kg) on the SETEM caused by THC (i.p., 1.0 mg/kg) and ethanol (i.p., 0.5, 1.0 and 2.0 g/kg) combination in male (a) and female (b) mice. c-e, Effects of strychnine (i.p., 1.0 mg/kg) (c), AM630 (i.p., 5.0 mg/kg) (d) and tranilast (i.p., 300 mg/kg) (e) on the SETEM caused by THC (i.p., 1.0 mg/kg) and ethanol (i.p., 1.0 and 2.0 g/kg) combination in mice. Stry: strychnine; Trani: Tranilast. f-i, Effects of AM251(f), strychnine (g), AM630(h) and tranilast (i) on AR latency of mice. j, Effects of AM251 (intra-4/5Cb., 5 μg /μl) on the SETEM caused by THC (i.p., 1.0 mg/kg) and ethanol (i.p., 1.0 and 2.0 g/kg) combination in mice. k, Effects of THC (intra-4/5Cb., 5 μg/μl) and ethanol (i.p., 1.0 g/kg) combination on AR latency in GlyRα1WT and GlyRα1S296A mice. l, Effects of THC (intra-4/5Cb., 5 μg/μl) and ethanol (i.p., 2.0 g/kg) combination on AR latency in GlyRα1WT and GlyRα1S296A mice. m, The AR latency of GlyRα1WT and GlyRα1S296A female mice receiving THC (i.p., 1.0 mg/kg) and ethanol (i.p., 0.5, 1.0 and 2.0 g/kg) combination. Values are represented as means ± s.e.m., n per group. Exact P values are shown. Statistical differences were determined by a one-way ANOVA followed by Tukey’s post hoc multiple-comparison test (a-j) or a two-way ANOVA followed by Tukey’s post hoc multiple-comparison test (k-m).

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