Extended Data Fig. 4: ABAT regulates T cell differentiation in vitro.
From: GAB functions as a bioenergetic and signalling gatekeeper to control T cell inflammation
a–d, Expression of indicated cytokines in indicated groups was determined by flow cytometry: a, c, d, data represents of n = 3 biologically independent samples, significance was calculated by one-way ANOVA with Tukey’s multiple comparisons test. ns, no significant differences. b, data represents of n = 4 biologically independent samples, unpaired Two-tail Student’s t-test. ns, no significant differences. e, Diagram of converting [13C4]-GABA (left) to downstream metabolites. Indicated metabolites were quantified by GC-MS (n = 3 biologically independent samples). Black dot: 12C; Blue dot: 13C derived from the indicated tracers. Numbers in the X-axis represent those of 13C atoms in given metabolites. Significance was calculated by unpaired Two-tail Student’s t-test. ABAT-OE: ABAT overexpression, α-KG: α-Ketoglutarate, M: mass spectrum.
