Fig. 3: ABAT enables diversion of GAB into the TCA cycle in T cells.
From: GAB functions as a bioenergetic and signalling gatekeeper to control T cell inflammation
a,b,c,d,e,g,Diagrams of the conversion of [13C4]GABA (a,b, left), [13C6]Arg (d, left), [13C4]Put (e, left) and [13C5]Gln (g, left) to downstream metabolites. Indicated metabolites in TH17 cells were quantified by GC–MS (n = 3 biologically independent samples) (right). Black dot, 12C; blue dot, 13C derived from the indicated tracers. Numbers along the x axis represent those of 13C atoms in the given metabolites. Significance was calculated by unpaired two-tailed Student’s t-test (a, b, d, e and g); NS, no significant differences. c,f, OCR of TH17 cells with the indicated treatments was determined by Seahorse. Data are representative of n = 16 independent biological samples from n = 3 independent biological experiments. Two-way ANOVA with Sidak’s multiple-comparisons test (c) and (f). α-KG, α-ketoglutarate; GDH, glutamate dehydrogenase.
