Fig. 4: Genetic ablation or pharmacological inhibition of ABAT suppresses TH17 but enhances iTreg cell differentiation.
From: GAB functions as a bioenergetic and signalling gatekeeper to control T cell inflammation
a,b, ABAT mRNA and protein levels were determined by qPCR and IB analysis (a, full-scan images are provided in as source data) or by flow cytometry (b); unpaired two-tailed Student’s t-test for a (n = 3 biologically independent samples). c, Expression of the indicated cytokines and CFSE dilution in the indicated groups determined by flow cytometry (WT, n = 5 independent biological samples from n = 5 independent biological experiments; Abat cKO, n = 5 independent biological samples from n = 3 independent biological experiments; WT plus Vig, n = 3 independent biological samples from n = 3 independent biological experiments). Two-way ANOVA with Sidak’s multiple-comparisons test. d–f, Hierarchical clustering analysis, gene set enrichment analysis (GSEA) and Ingenuity Pathway Analysis (IPA) of a list of inflammatory genes performed by using RNA-seq data of CD4+ T cells that were cultured under TH0 culture conditions and collected at 36 h after activation (n = 3 independent biological samples). In d, heatmaps represent the log-transformed value of the quantity (see colour scale). In f, the orange dotted line along the x axis indicates the cut-off value (P = 0.05). The complete data are provided as source data. g, As illustrated by the experimental scheme (top), the expression of the indicated proteins in CNS-infiltrating CD4+ T cells isolated from EAE animals was determined by flow cytometry (n = 3 independent biological samples from n = 2 independent biological experiments). h,j, EAE clinical scores in the indicated groups evaluated daily from mice of the indicated genotypes (n = 6 independent biological samples from n = 2 independent biological experiments in h and n = 5 independent biological samples from n = 3 independent biological experiments in j); significance was calculated by unpaired two-tailed Student’s t-test. i,k, The expression of the indicated markers in CNS-infiltrating T cells determined by flow cytometry (n = 5 independent biological samples in i and n = 3 independent biological samples in k). PTX, paclitaxel; NES, normalized enrichment score; FDR, false discovery rate.
