Fig. 6: GAB exerts both bioenergetic and receptor signalling-mediated control of T cell differentiation.
From: GAB functions as a bioenergetic and signalling gatekeeper to control T cell inflammation
a, Schematic diagram of GAB catabolism through the TCA cycle. b, OCR of TH17 cells with the indicated treatments determined by Seahorse (n = 16 independent biological samples from n = 3 independent biological experiments); significance was calculated by two-way ANOVA with Sidak’s multiple-comparisons test. c,d, Expression of IL-17A in the indicated groups determined by flow cytometry (n = 3 independent biological samples); significance was determined by one-way ANOVA with Tukey’s multiple-comparisons test. e, Left, experimental diagram of the competitive Treg suppression assay. Right, CFSE dilution was determined by flow cytometry (n = 3 independent biological samples); significance was determined by two-way ANOVA with Sidak’s multiple-comparisons test. f, Schematic conceptual model in which GAB generated by Gln and Arg can regulate T cell differentiation by entering the TCA cycle or being exporting into the extracellular environment and acting on GABAA-R. Tconv, conventional T cells.
