Extended Data Fig. 2: Aldometanib binds aldolase at the K230 residue.
From: The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPK
a, Typical spectrograms of K230 residues with (right) and without (left) aldometanib conjugation. Some 1 μg of rabbit aldolase was incubated with 50 μM aldometanib for 2 h, followed by SDS-PAGE and analysis of covalent modifications through mass spectrometry. b, In silico docking results showing that the groove near the K230 residue is required for aldometanib binding. c, Enzymatic activities of ALDOA (100 nM, bacterially expressed and purified) carrying mutations on residues that may be responsible for aldometanib binding. Results are shown as mean ± s.d.; n = 4 (ALDOA WT) or 3 (others) biological replicates for each condition. Experiments in this figure were performed three times.
