Fig. 2: Harmonized annotation of hypothalamus cell types.
From: HypoMap—a unified single-cell gene expression atlas of the murine hypothalamus
a, A circular hierarchical tree of clusters of HypoMap. The first 5 levels with up to 185 clusters are shown, highlighting the diversity of hypothalamic cells when combining data across regions. Individual clusters at levels 4 and 5 are named with the most informative marker gene, given as edge labels. The inner (red) circular heatmap depicts the percentage contribution of each dataset to the clusters at the lowest tree level. The middle heatmap (blue) depicts the relative percentage contribution of each cluster at the lowest tree level to the total cell number. The scale is limited to 2%. The outer ring depicts the most likely region of origin (R) for each neuron cluster on the lowest level of the displayed tree. If support was insufficient for a cluster, no region was assigned, and the cluster was colored gray (see Methods). b,c, Dot plots displaying marker genes used for annotating the clusters at level 4 (C66) of the tree in a. For clusters with a proper name (for example, ‘Astrocytes’), the most specific gene that would have been used for annotation is included. Dot color corresponds to average log-normalized expression levels of each gene in a cluster and dot size to the percentage of cells expressing a marker in the cluster. b, Neuronal cell types. c, Non-neuronal cell types. See also Supplementary Tables 5 and 6.
