Fig. 6: Projection of new data.
From: HypoMap—a unified single-cell gene expression atlas of the murine hypothalamus
a, HypoMap UMAP colored by cluster level 3 (C25) and overlaid with the projected ‘locations’ of cells from Romanov et al.20. Even clusters represented by only few cells in the query dataset can be accurately embedded into the reference. b, Probability scores (see Methods) of projection accuracy of Romanov et al.20 cells from a. High scores indicate high confidence in the projection, which is the case for most cells. c–f, Enrichment of bacTRAP signatures of specific neuronal population on HypoMap clusters using rank-biased overlap (RBO). RBO scores per cluster (C286) are shown as small bars relative to the highest score of each signature enrichment. The UMAP shows the expression level of the marker gene used in the bacTRAP experiment in HypoMap. In e and f, it shows the cells that express the combination of marker genes in orange (square root of product of expression levels). The corresponding cluster names for each unique ID in the figure can be found in Supplementary Table 3. c, AgRPCre neurons are enriched in C66-46: Agrp.GABA-4 subclusters. d, PomcCre neurons are highly enriched in the C66-19: Pomc.GLU-5 subclusters, with medium scores in C66-46: Agrp.GABA-4. e, PomcDreLeprCre neurons are most enriched in C286-75: Anxa2.Pomc.GLU-5, which expresses Lepr. f, PomcDreGlp1rCre neurons are most enriched in C286-77: Ttr.Pomc.GLU-5, which expresses Glp1r. Also see Supplementary Table 19.
