Extended Data Fig. 4: Macrophages respond to Ftmt overexpression by up-regulating iron uptake.
(a-q) Male control and Mac-FtmtTG mice were fed chow dox for 2 weeks. (a) Labile iron pool (LIP) in mitochondrial and cytosolic compartments of peritoneal macrophages (n = 4). (b) mRNA levels of iron metabolism genes relative to control in peritoneal macrophages (control n = 5, TG n = 7). (c) CD91 (n = 8), (d) CD163 (n = 8), (e) Ferritin (n = 8), (f) Ferroportin (n = 4) and (g) MitoNEET (n = 8) protein expression relative to β-actin in peritoneal macrophages (n = 8). (h) Representative Western blots chosen from 2 independent experiments. (i) mRNA levels of inflammation markers relative to control in peritoneal macrophages (control n = 5; TG n = 7). (j-l) Flow cytometric measurement of total, M1-like and M2-like macrophages in eWAT (n = 6). (m) Body weight (n = 6). (n-o) Oral glucose tolerance test (n = 6). (n) Blood glucose levels (n = 6). (o) Serum insulin levels (control n = 7, TG n = 9). (p) Blood glucose levels during insulin tolerance test (control n = 6, TG n = 5). (q) Serum triglyceride levels during triglyceride clearance (n = 6). Significance in (a-g, i-m) between control and Mac-FtmtTG was calculated using a two-tailed Student’s t-test. Significance in (n-q) was calculated using a 2-way ANOVA with Sidak’s post-test for multiple comparisons. Error bars represent mean ± S.E.M. * (P < 0.05), ** (p < 0.01), *** (p < 0.0001), **** (p < 0.00001).
