Extended Data Fig. 7: Eliminating MitoNEET in macrophages during HFD promotes insulin resistance and inflammation.
(a-k) Six-week-old male control and Mac-MitoNEETKO mice were fed HFD dox for 7 weeks. (a) Breeding strategy. (b) MitoNEET protein expression relative to β-actin in peritoneal macrophages (n = 3). (c) Representative Western blots (n = 3). (d) Body weight (control n = 9, TG n = 5). (e) Tissue weights (control n = 9, TG n = 5). (f-g) Oral glucose tolerance test. (f) Blood glucose levels (control n = 5, TG n = 3). (g) Serum insulin levels (control n = 9, TG n = 5). (h) Blood glucose levels during insulin tolerance test (control n = 9, TG n = 5). (i-k) Flow cytometric measurement of total, M1-like and M2-like macrophages in eWAT (control n = 5, TG n = 3). Significance in (b, e, i-k) between control and Mac-MitoNEETKO was calculated using a two-tailed Student’s t-test. Significance in (d, f-h) was calculated using a 2-way ANOVA with Sidak’s post-test for multiple comparisons. Error bars represent mean ± S.E.M. * (P < 0.05), ** (p < 0.01), *** (p < 0.0001), **** (p < 0.00001).
