Extended Data Fig. 9: Validation of eWAT transplantation to wild-type mice.
(a-i) Six-week-old male control and Mac-MitoNEETTG mice were injected with PKH67 dye before transplantation of their eWAT to wild-type mice (n = 5). Recipient mice were allowed to recover 3 weeks and then fed HFD dox for 6 weeks. (a-b) Flow cytometric measurement of PKH67 fluorescence in (a) graft and (b) recipient eWAT (n = 5). (c-d) mRNA levels of Cisd1, Ftmt, and Tfam relative to control in (c) graft and (d) recipient eWAT (n = 5). (e-g) Flow cytometric measurement of total, M1-like, and M2-like macrophages in recipient eWAT (n = 5). (h-i) Wild-type mice were transplanted with either control or Mac-FtmtTG eWAT. Transplanted mice were allowed to recover 3 weeks and then fed HFD dox for 6 weeks. (h-i) mRNA levels of Cisd1, Ftmt and Tfam relative to control in (c) graft and (d) recipient eWAT (control n = 7, TG n = 8). Significance in (a-i) between control and transgenic fat-transplanted animals was calculated using a two-tailed Student’s t-test. Error bars represent mean ± S.E.M. * (P < 0.05), ** (p < 0.01), *** (p < 0.0001), **** (p < 0.00001).
