Extended Data Fig. 1: Validation of MitoNEET overexpression in macrophages.
(a-j) Six-week-old male control and Mac-MitoNEETTG mice were fed chow dox for 2 weeks. (a) Breeding strategy. (b) Cisd1 mRNA levels relative to control in monocytes (control n = 4, TG n = 3), ATMs (n = 3), and peritoneal macrophages (n = 6). (c) MitoNEET protein expression relative to β-actin in peritoneal macrophages (n = 6). (d) Representative Western blots chosen from 2 independent experiments. (e-f) Mitochondrial respiration in peritoneal macrophages (control n = 3, TG n = 5). (g) Mitochondrial electron transport chain protein expression relative to prohibitin in peritoneal macrophages (n = 6). (h) Representative Western blots. (i-j) Glycolysis stress test in peritoneal macrophages (control n = 6, TG n = 6). Significance in (b-c, f-g, j) between control and Mac-MitoNEETTG was calculated using a two-tailed Student’s t-test. Significance in (e, i) was calculated using a 2-way ANOVA with Sidak’s post-test for multiple comparisons. Error bars represent mean ± S.E.M. * (P < 0.05), ** (p < 0.01), *** (p < 0.0001), **** (p < 0.00001).
