Extended Data Fig. 5: Brown adipocytes (BA) or rNrg4 reduced apoptosis, adhesion and inflammatory responses in MAECs.
(A) Representative images of apoptotic cells in MAECs. (B) The percentage analysis of apoptotic cells of (A). (C and D) Expressions of bax, bcl-2 and cleaved-caspase 3 in MAECs. (E) The mRNA levels of adhesion molecules and inflammatory cytokines in lysate of MAECs. (F) MTT assay for the optimum intervention condition of ox-LDL. (G) MTT assay for the optimum treatment condition of rNrg4. (H) Representative images of apoptotic cells by flow cytometry assay in MAECs and the percentage analysis of apoptotic cells (I). (J) Expressions of bax, bcl-2 and cleaved-caspase 3 in MAECs. (K) MEACs permeability. (L) The mRNA levels of adhesion molecules and inflammatory cytokines in lysate of MAECs. The BA were obtained from WT or KO mice. The co-culture BA experiment was a noncontact co-cultured assay through a transwell 24-well plate for 48 h treatment. The MAECs from WT mice were pretreated with rNrg4 100 ng/ml for 48 h and ox-LDL 100 μg/ml for 12 h. Each experiment was repeated 5 times. Statistical significance was calculated using one-way ANOVA with Tukey’s multiple-comparison test. Data were shown as mean ± SEM. *P < 0.001.
