Fig. 7: Nrg4 inhibited NF-κB signal in vivo and in vitro.
AAV-Nrg4 or AAV-Zsgreen at a dose of 1 × 1012 viral genomes were injected into the BAT in the interscapular region of KO mice at aged 6 weeks and then were fed WD or NCD for 12 weeks. a, KEGG pathway analysis indicated the altered function of NF-κB signaling pathway. b, Heat map of the NF-κB pathway and apoptosis-associated genes. c, The expression levels of NF-κB signaling in MAECs of KO and WT mice. d, The expression levels of NF-κB signaling in MAECs of KO and WT mice with or without the overexpression of Nrg4. e, Quantitative analysis of c (n = 6). f, Quantitative analysis of d (n = 6). g, The expression of p65 and P-p65 in MAECs nucleus or cytoplasm under the presence or absence of rNrg4. h, Quantitative analysis of g (n = 5 independent experiments). I, The p65 nuclear translocation in MAECs. Scale bar, 50 μm. P values were calculated by two-sided Student’s t-test or one-way ANOVA with Tukey’s multiple-comparison test. Data are shown as mean ± s.e.m. *P < 0.001.
