Extended Data Fig. 9: Negligible effects of food-odor stimulation during fasting on metabolic activity in the adipose tissues and brain and gene expression in the liver after refeeding.
From: Food odor perception promotes systemic lipid utilization
C57BL/6 J mice fasted for 24 h were stimulated with NCD odor or none for 1 h, and then refed NCD for 3 h. a, Metabolomic profiles in the epididymal white adipose tissue (eWAT), brown adipose tissue (BAT), and whole brain under the 3-h refeeding condition. OAA: oxaloacetic acid, α-KG: α-ketoglutarate. n = 8 (None) and n = 9 (Odor) biological replicates. b, Insulin tolerance test (ITT) under the 3-h refeeding condition. Insulin (0.5 unit/kg, i.p.) was injected at 0 min. n = 7 biological replicates. c, Expression levels of genes related to de novo lipogenesis and cholesterol synthesis in the liver under the 3-h refeeding condition. Srebp1c: sterol regulatory element-binding protein 1c, Acc: acetyl-CoA carboxylase, Fasn: fatty acid synthase, Mtp: microsomal triglyceride transfer protein, Atgl: adipose triglyceride lipase. n = 16 (None) and n = 18 (Odor) biological replicates. **P < 0.01 determined by unpaired two-tailed t-test. Data are presented as mean ± s.e.m.
