Extended Data Fig. 8: Brain ECs show obesity-induced changes in metabolic, cell junction and solute carrier genes.
(a) Top 5 enriched genes in each of the neural EC clusters. (b) Shifts in neural EC clusters. Quantification of the shifts is provided in the lower bar graph, with changes expressed on a log2 scale. (c) Comparison of obesity-associated gene expression changes across neural EC subtypes. Any gene showing a |log(FC)| > 0.1 in any cluster was used to collate the list of genes used for these analyses. Pearson’s r-value for each comparison is provided. (d) Heatmap depicting obesity-induced gene expression changes in mitochondrial encoded members of the electron respiration chain. (e) UMAPs depicting enrichment of Sgms1 and Degs2 mRNA in ECs across all organs. Obesity-associated gene expression changes in neural ECs are shown. (f) Enrichment (z-scores) of tight junctions (blue), adherent junctions (green), gap junctions (black) and leukocyte adhesion molecules (red) across neural EC clusters. (g) Heatmap showing the enrichment (z-scores) of SLC transporters across neural EC clusters. The Slc genes enriched in fenestrated EC population are indicated by the red box. (h) Gene expression changes in select Slc genes in fenestrated ECs in obesity. (i) Representative mages of Dextran 3 kDa (FITC) and Dextran 70 kDa (Texas red) in the choroid plexus of obese and control animals. ECs were immunostained with CD31 (purple). All images were taken with the same exposure and laser power. Quantifications are provided in Fig. 2r. N = 5 animals per group. Scale bars represent 20 µm. FC – fold change. Differential expression from scRNA-seq data is expressed on a natural log (loge) scale.
