Extended Data Fig. 3: Mapping inputs of GluLHA neurons and GABALHA neurons.
From: A neural circuit for the suppression of feeding under persistent pain
a, Schematic of viral injection. b, Representative confocal image of the starter neurons (yellow) within the LHA of VgluT2-Cre mice. Scale bar, 200 µm. The white box depicting the area shown in the box of the LHA. Scale bar, 10 µm. c, Representative confocal images of DsRed+ neurons expression in the MPA, PVN, CeA and DRD of mice injected with helper and RV-EvnA-DsRed into the LHA. Scale bars, 200 µm. d, Schematic of viral injection. e, Representative confocal images of the starter neurons (yellow) within the LHA of Vgat-Cre mice. Scale bar, 200 µm. The white box depicting the area shown in the box of the LHA. Scale bar, 10 µm. f,g, Representative confocal images of RV-DsRed expression in the ACC and in the PVN, CeA, and RtTg. Scale bars, 200 µm. MPA, medial preoptic area. PVN, paraventricular hypothalamic nucleus. CeA, amygdala. DRD, dorsal raphe nucleus. RtTg, reticulotegmental nucleus of the pons.
