Extended Data Fig. 5: The effects of activation of GluLHA neurons on food intake.
From: A neural circuit for the suppression of feeding under persistent pain
a, Schematic of viral injection and chemogenetic activation design. b, Representative confocal image of AAV-DIO-hM3Dq-mCherry expression in the LHA of VgluT2-Cre mice. Scale bar, 200 µm. c, Whole-cell recording showing the effects of CNO on AAV-DIO-mCherry- or AAV-DIO-hM3Dq-mCherry-expressing GluLHA neurons (mCherry, n = 3 cells from 3 mice; hM3Dq-mCherry, n = 4 cells from 4 mice). d, Example recording of spontaneous spikes (left) and statistical data (right) showing the GluLHA firing rates in VgluT2-Cre mice injected with AAV-DIO-mCherry or AAV-DIO-hM3Dq-mCherry (mCherry, n = 53 cells from 5 mice; hM3Dq-mCherry, n = 55 cells from 5 mice). e, Effects of chemogenetic activation of GluLHA neurons on food intake in mice deprived of food for 24-hour (mCherry, n = 12 mice; hM3Dq-mCherry, n = 11 mice; F1,21 = 14.67, P = 0.001). f, Spatial location heatmaps from free feeding tests of mice deprived of food for 24-hour and chemogenetic activation of GluLHA neurons. g, Quantitative analysis of time in the food zone (%) (left) and food intake (right) for mice as indicated in (f) (n = 11 mice per group; left, t20 = 6.175, P < 0.0001; right, t20 = 3.607, P = 0.002). h, Effects of chemogenetic activation of GluLHA neurons on pain threshold in CFA 10 d mice (mCherry, n = 12 mice; hM3Dq-mCherry, n = 11 mice). Significance was assessed by two-way repeated-measures ANOVA with post hoc comparison between groups in (c and e), and two-tailed unpaired Student’s t test in (d,g and h). All data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.
