Extended Data Fig. 10: Activation of LHA-projecting ACC neurons is sufficient for suppression of food intake.
From: A neural circuit for the suppression of feeding under persistent pain
a, Schematic chemogenetic activation design. b, Representative confocal image of AAV-DIO-hM3Dq expression in the ACC of B6 (C57) mice. Scale bar, 200 µm. c, Example recording of spontaneous spikes (left) and statistical data (right) showing the GluACC firing rates in B6 (C57) mice injected with AAV-DIO-mCherry or AAV-DIO-hM3Dq-mCherry (mCherry, n = 57 cells from 6 mice; hM3Dq-mCherry, n = 57 cells from 6 mice). d, Representative confocal images (left) and statistical analysis (right) showing c-Fos-positive neurons in the Arc of mice injected with AAV-DIO-mCherry or AAV-DIO-hM3Dq-mCherry into the ACC (n = 6 slices from 6 mice per group; t10 = 11.09, P < 0.0001). Scale bars, 200 µm. e, Representative confocal images (left) and statistical analysis (right) showing that c-Fos-labeled neurons in the Arc predominantly colocalized with the POMC antibody (n = 6 slices from 6 mice). Scale bar, 20 µm. f, Chemogenetic activation of the LHA-projecting ACC neurons significantly decreased food intake (mCherry, n = 12 mice; hM3Dq-mCherry, n = 11 mice; t21 = 5.707, P < 0.0001). Significance was assessed by two-tailed nested t-test in (c), and two-tailed unpaired Student’s t test in (d and f). All data are presented as the mean ± SEM. * P < 0.05, *** P < 0.001.
