Extended Data Fig. 6: Maternal tryptophan supplementation does not rescue behavior in female mHFD offspring.
From: Maternal diet disrupts the placenta–brain axis in a sex-specific manner
a, Maternal dietary tryptophan enrichment does not impact adult female offspring midbrain serotonin levels (n = 11 mLFD, 11 mLFD+Trp, 9 mHFD, 10 mHFD+Trp offspring from 6 mLFD, 9 mLFD+Trp, 7 mHFD, and 8 mHFD+Trp litters). b-h, Maternal dietary tryptophan enrichment does not rescue mHFD-dependent changes to neonatal female ultrasonic vocalizations (n = 19 mLFD, 28 mLFD+Trp, 23 mHFD, 22 mHFD+Trp offspring from 6 mLFD, 8 mLFD+Trp, 8 mHFD, and 9 mHFD+Trp litters). i, Maternal tryptophan enrichment increases female offspring sucrose preference regardless of maternal dietary fat intake (n = 8 mLFD, 8 mLFD+Trp, 8 mHFD, 11 mHFD+Trp from 3 mLFD, 5 mLFD+Trp, 4 mHFD, and 6 mHFD+Trp litters) j-m, Maternal tryptophan enrichment influence on juvenile social preference (n = 8 mLFD, 11 mLFD+Trp, 6 mHFD, and 10 mHFD+Trp offspring from 3 mLFD, 5 mLFD+Trp, 4 mHFD, and 3 mHFD+Trp litters). n-q, Maternal tryptophan enrichment influence on open field behavior (n = 13 mLFD, 11 mLFD+Trp, 9 mHFD, and 12 mHFD+Trp offspring from 4 mLFD, 4 mLFD+Trp, 4 mHFD, and 4 mHFD+Trp litters). Data are mean ± s.e.m. except for the box plot (c) where whiskers are min. to max., hinges of boxes are 25th and 75th percentiles, and the middle line is the median; P values are derived from 2-way ANOVA (fat content x tryptophan content; b, c, d, e, f, g, h, i, n, o) or one-sample t-tests assessing difference from chance (50%; j; &P < 0.01). n.s. not significant.
