Extended Data Fig. 8: mHFD-induced TLR4-dependent inflammation drives offspring behavior changes.
From: Maternal diet disrupts the placenta–brain axis in a sex-specific manner
a, USV metrics from male and b, female control and cKO mLFD and mHFD offspring (n = 15 male mLFD control and 8 cKO from 5 litters and 14 male mHFD control and 14 cKO from 6 litters; n = 9 female mLFD control and 12 cKO from 7 litters and 6 female mHFD and 10 cKO from 5 litters). c, Total liquid consumption in mLFD and mHFD control and cKO males (n = 11 mLFD, 10 mLFD cKO, 11 mHFD, and 10 mHFD cKO from 5 mLFD and 6 mHFD litters) d, Social preference in male mLFD and mHFD control and Tlr4 cKO offspring (n = 10 mLFD, 10 mLFD cKO, 12 mHFD, 10 mHFD cKO from 5 mLFD and 6 mHFD litters) e, Sucrose preference in female mLFD and mHFD control and Tlr4 cKO offspring (n = 7 mLFD, 7 mLFD cKO, 7 mHFD, 10 mHFD cKO from 5 mLFD and 4 mHFD litters) f, Social preference metrics in female mLFD and mHFD control and Tlr4 cKO offspring (n = 9 mLFD, 6 mLFD cKO, 7 mHFD, and 6 mHFD cKO from 5 mLFD and 5 mHFD litters). Data are mean ± s.e.m. except for the box plots (a, b) where whiskers are min. to max., hinges of boxes are 25th and 75th percentiles, and the middle line is the median; P values are derived from two-way ANOVA (a, b, d, f) or one-sample t-tests assessing difference from chance (50%; d, e; #P < 0.0001, †P < 0.001, &P < 0.01, ^P < 0.05).
