Extended Data Fig. 2: SARS-CoV-2 instigates tissue hypoxia and VEGF expression in mice.
From: COVID-19 instigates adipose browning and atrophy through VEGF in small mammals
(a) Histological and immunohistochemical analyses of lung tissues of NI- and day-6-post SC-infected mice by staining with H&E, HIF1α and CA9. Tissue sections were counterstained with DAPI (blue). Quantification of HIF1α and CA9 positive signals in lung tissues (n = 8 random fields per group). Quantification of Car9 and Hif1a mRNA and HIF1α and CA9 protein levels by qPCR (n = 8 samples per group) and Western immunoblot (n = 6 samples per group) in lung tissues. (b) Quantification of Car9 and Hif1a mRNA levels by qPCR in BAT of NI- or day-6-post SC-infected mice (n = 8 samples per group). (c) Quantification of HIF1α and CA9 protein levels by Western immunoblot in BAT of NI- or day-6-post SC-infected mice. β-actin served as internal controls (n = 6 samples per group). (d) qPCR quantification of Vegf mRNA levels in BAT of NI- or day-6-post SARS-CoV-2-infected mice (n = 8 samples per group). (e) CD31 and Ki67 staining and quantification of microvessels in BAT of NI- or day-6-post SC-infected mice. Tissue sections were counterstained with DAPI (blue). CD31 and Ki67 double positive signals of BAT were quantified (n = 8 random fields per group). Data are presented as means ± s.e.m. Statistical analysis was performed using two-sided unpaired t-tests. NI = non-infected. SC = SARS-CoV-2. Scale bar = 50 μm.
