Extended Data Fig. 6: Ablation of HK2 did not affect microglial density and morphology in the adult stage.
(a) A scheme of the experimental timeline for the analysis of microglial density and morphology from Hk2fl/fl and Hk2-cKO mice at 1 week after TAM administration. (b) Representative micrographs (from 3 independent experiments) showing the Iba1+ microglia and proliferative (BrdU+) microglia in adult Hk2fl/fl and Hk2-cKO mouse brains at 1 week after TAM administration. (c, d) Quantification of microglial number (c) and percentage of BrdU+ microglia (d) in the cortex from Hk2fl/fl and Hk2-cKO mice. n = 3 mice per group. (e) A scheme of the experimental timeline for analysis of microglial density and morphology from Hk2fl/fl and Hk2-cKO mice at 4 weeks after TAM administration. (f, g) Flow cytometry analysis and quantification of CD11b+ CD45low microglia in adult mice at 4 weeks after TAM administration. n = 3 mice per group. (h) Representative micrographs (from 3 independent experiments) showing 3D reconstruction of microglia from Hk2fl/fl and Hk2-cKO mice in the cortex using Imaris. (i) Scholl analysis of all reconstructed cells. n = 3 mice per group (total 36 cells). (j-l) Quantification of microglial morphology in the cortex from Hk2fl/fl and Hk2-cKO mice by Sholl analysis. n = 3 mice per group (total 36 cells). TAM, tamoxifen; D, day. Data are presented as means ± SEM. Two-tailed Student’s t-tests for c, d, f, g, j-l, *p < 0.05.
