Extended Data Fig. 7: Ablation of HK2 did not affect Hk1 and Hk3 levels. | Nature Metabolism

Extended Data Fig. 7: Ablation of HK2 did not affect Hk1 and Hk3 levels.

From: Dual roles of hexokinase 2 in shaping microglial function by gating glycolytic flux and mitochondrial activity

Extended Data Fig. 7

(a) The expression of Hk1, Hk2 and Hk3 in FACS-sorted microglia from Hk2fl/fl and Hk2-cKO mice at adult. n = 4 or 3 mice in Hk2fl/fl or Hk2-cKO groups, respectively. (b-d) The expression of Hk1, Hk2 and Hk3 in sorted microglia during repopulation in Hk2fl/fl and Hk2-cKO mice. n = 1481 or 264 cells for HomoMG, 162 or 264 cells for Precursor MG, 121 or 91 cells for G1/S MG, 200 or 108 cells for G2/M MG in Hk2fl/fl or Hk2-cKO groups, respectively. (e) Quantitative analyses of Hk1, Hk2, Hk3 and Cx3cr1 expression in the brain tissues from Hk2fl/fl and Hk2-cKO mice. n = 4 or 3 mice in Hk2fl/fl or Hk2-cKO groups, respectively. (f) CD11b+CD45low microglia and CD11b-CD45− non-microglial cells sorting from Hk2fl/fl and Hk2-cKO mice by flow cytometry. (g) Quantitative analyses of Hk2 and Cx3cr1 expression in CD11b−CD45− non-microglial cells and CD11b+CD45low microglia from Hk2fl/fl and Hk2-cKO mice. n = 3 mice for microglia and non-microglial cells or 4 mice for microglia in Hk2fl/fl or Hk2-cKO groups, respectively. Data are presented as means ± SEM. Two-tailed student t-tests for a, e, g. Wilcoxon rank sum test and Bonferroni correction for b-d, *p < 0.05, **p < 0.01 and ***p < 0.001.

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