Extended Data Fig. 8: A comparison of repopulated microglia in Hk2fl/fl and Hk2-cKO mice. | Nature Metabolism

Extended Data Fig. 8: A comparison of repopulated microglia in Hk2fl/fl and Hk2-cKO mice.

From: Dual roles of hexokinase 2 in shaping microglial function by gating glycolytic flux and mitochondrial activity

Extended Data Fig. 8

(a) Pseudotime trajectory was constructed using Monocle 2 by re-ordering microglia population and single cells on the trajectory. Each microglia on the minimum spanning tree (MST) were colored by pseudotime. (b) Pseudotime trajectory was constructed and each microglia on the MST were colored by annotated microglial cell types during repopulation in Hk2fl/fl and Hk2-cKO mice. (c) The number of cells in different subsets of microglia from Hk2fl/fl and Hk2-cKO mice. (d) t-SNE plots of Hk2, Cx3cr1, Cdkn1a and Slfn5 gene expression between Hk2fl/fl and Hk2-cKO mice. n = 1481 or 264 cells for HomoMG, 162 or 264 cells for Precursor MG, 121 or 91 cells for G1/S MG, 200 or 108 cells for G2/M MG in Hk2fl/fl or Hk2-cKO groups, respectively. The color key indicates the expression levels.

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