Extended Data Fig. 9: Analyses of microglial number, phagocytosis, motor behavior and inflammation in Hk2^fl/fl and Hk2-cKO mice before and after stroke.

(a) Representative micrographs (from 3 independent experiments) showing the density of microglia in the peri-infarct zone from Hk2^fl/fl and Hk2-cKO mice at 3 and 7 Dpi. Dashed lines and dashed boxes indicate the border of ischemic core and regions of higher magnification, respectively. Rotarod (b, c) and open field (d, e) tests of adult Hk2^fl/fl and Hk2-cKO mice. n = 10 mice per group. (f) Representative micrographs (from 3 independent experiments) showing CD68⁺ labeling in Iba1⁺ microglia in the peri-infarct zone from Hk2^fl/fl and Hk2-cKO mice under sham and ischemic conditions (3 Dpi) and quantification of microglial volume and CD68⁺ volume in microglia (g, h). Dashed boxes show regions of higher magnification of microglia. n = 30 cells from 3 mice per group. RT-qPCR analysis of Tnfα, Il-6 and Il-1β levels (i) and Ifitm3, Stat1 levels (j) in the infarcted tissues at 1 Dpi. n = 5 mice per group for sham-operated animals or 6 mice per group for ischemic animals. RPM, rotations per minute; Dpi, days post ischemia. Data are presented as means ± SEM. Two-tailed student t-tests for b-e. Two-way ANOVA followed by Bonferroni’s post hoc tests for g, h, *p < 0.05, **p < 0.01 and ***p < 0.001.

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