Extended Data Fig. 8: Characterization of hPSC-islet grafts of Monkey 5 and 6 at 12 weeks after sub-rectus sheath transplantation.
a-b, Representative immunofluorescence staining of PCSK1 (a) and PCSK2 (b) in subanterior rectus sheath grafts and human islets. Scale bar, 50 μm. Similar results were obtained from five independent grafts. c, Vessel density in hPSC-islet grafts and native human islets (n = 10 independent grafts). Two-tailed t-test was used to determine significance between groups and P value was indicated above the bars. d, Representative immunohistochemical staining of T cell marker CD3, B cell marker CD20 and macrophage marker CD68 in subanterior rectus sheath grafts (left). Monkey spleen or liver sections were used as control. The quantitative analysis of immunological infiltration in hPSC-islet grafts (n = 10 independent grafts) (right). Scale bars, 100 μm. Data presented as mean ± SEM. Two-tailed t-test was used without adjustments for multiple comparisons to determine significance between groups and P value was indicated above the bars.
