Extended Data Fig. 2: The effect of EVs release blockade or EVs supplementation on the host defense response to S.Tm infection.
From: Humoral regulation of iron metabolism by extracellular vesicles drives antibacterial response
a-c, To evaluate the effects of EVs release blockade on iron homeostasis, uninfected or S.Tm-infected mice were pretreated with GW4869 to block EVs release. a, Western blot analysis of FPN1 and FTH1 expressions in liver or spleen. Experiments were repeated three times and representative images are shown. b, The iron levels in liver or spleen at 12 hours after S.Tm infection. n = 6 mice. c, The viable count of S.Tm in liver and spleen at 12 hours after S.Tm infection. n = 6 mice. d,e, GW4869-pretreated S.Tm-infected mice were injected with EVs derived from uninfected mouse serum (Serum EVs group) or EVs derived from S.Tm-infected mouse serum [Serum(S.Tm)-EVs group]. d, The viable count of S.Tm in the liver and spleen. n = 5 mice. e, Representative fluorescence images of LPS (red) in the liver (upper) and spleen (bottom) and quantitative analysis of the percentage of LPS + area in liver or spleen cells. Scale bar, 50 μm. n = 3 biologically independent samples. For b-e, data are represented as the mean ± s.d. For b-e, statistical significance was assessed by one-way ANOVA with Tukey’s post-hoc test.
