Extended Data Fig. 3: S.Tm infection affects iron homeostasis in BMDM.
From: Humoral regulation of iron metabolism by extracellular vesicles drives antibacterial response
a, Flow cytometric analysis of the expressions of macrophage surface marker F4/80 and CD11b on BMDM. b, c, BMDM were treated with S.Tm in the absence or presence of GW4869 for 24 hours to determine the iron homeostasis. b, Western blot analysis of expressions of iron-related proteins TfR, CD91, CD163, FTH1, and FPN1. Experiments were repeated three times and representative images are shown. c, The iron content in BMDM. n = 5 biologically independent samples. For c, data are represented as the mean ± s.d. For c, statistical significance was assessed by one-way ANOVA with Tukey’s post-hoc test.
