Extended Data Fig. 1: The characterization of S.Tm-infected mice and serum EVs biodistribution in vivo.
From: Humoral regulation of iron metabolism by extracellular vesicles drives antibacterial response
a-c, The C57BL/6 J mice were intraperitoneally administrated with S.Tm and blood and tissues samples were collected for further analysis. a, The viable counts of S.Tm in the blood at 24 hours after infection. n = 6 mice. b, Serum (left), hepatic (middle), and splenic (right) iron levels in mice at 24 hours after infection. n = 6 mice. c, The concentration of EVs in serum within 24 hours after infection. n = 3 mice per time point. d, Ex vivo fluorescence images of various organs in mice systemically injected with DiR-labeled serum EVs. n = 3 mice. e, f, Confocal microscopy images showing the uptake of PKH26-labeled EVs (red) by F4/80+ cells (green) (e) or Macro+ cells (green) (f) in liver or spleen. Scale bar, 10 μm. n = 3 biologically independent samples. For a-c, data are presented as the mean ± s.d. For a-c, statistical significance was assessed by unpaired two-sided Student’s t-test.
