Extended Data Fig. 9: Phospho-deficient Wdr24^S155A knock-in mice show relatively high mTORC1 activity under fasting.

a, Representative images of hematoxylin and eosin (H&E) (scale bar, 50 μm), p-S6(S40/244) (scale bar, 100 μm) staining of the heart section (n = 3) of Wdr24^+/+and Wdr24^A/A littermates. The mice were fasted for 24 h, or fasted and refed for 2 h. b, IB analysis of WCLs derived from Wdr24^+/+or Wdr24^A/A mouse livers. The mice were fasted for 24 h, or fasted and refed for 2 h. n = 3 mice. c, Representative images of hematoxylin and eosin (H&E) (scale bar, 50 μm), p-S6(S240/244) staining (scale bar, 100 μm; insets magnification=1.5) of the kidney section (n = 3) of Wdr24^+/+and Wdr24^A/A littermates. The mice were fasted for 24 h, or fasted and refed for 2 h. d, IB analysis of WCLs derived from Wdr24^+/+or Wdr24^A/A mouse kidneys. The mice were fasted for 24 h or fasted and refed for 2 h. n = 3 mice. e,f, IF analysis of the kidney section of Wdr24^+/+and Wdr24^A/A littermates (e). Scale bar, 20 μm. The mice were fasted for 24 h, or fasted and refed for 2 h. The pS6 (240/244) intensity was quantified via Image J (f). n = 7, 6, 6, 6. P = 1.27E-06, 5.95E-07, 0.01. Data are mean ± s.d., two-tailed t-test. *P < 0.05, ***P < 0.001.

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