Extended Data Fig. 10: The WDR24-S155A mutation partially regulates mTORC1 lysosome localization under glucose starvation.
a, IB analysis of WCLs derived from Wdr24+/+or Wdr24A/A MEFs. The cells were deprived of amino acids for 60 min and restimulated with amino acids for 10 min as indicated. Representative image shown, n = 2. b, IF analysis of Wdr24+/+or Wdr24A/A MEFs. Indicated cells were deprived of glucose for 60 min and restimulated with glucose for 10 min as indicated. Scale bar, 10 μm. c, The imaging data in (b) were quantified. n = 14, 12, 12, 14. P = 4.74E-09, 1.57E-08, 0.003. Data are the mean ± s.d., two-tailed t-test. **P < 0.01, ***P < 0.001. d, IF analysis of Wdr24+/+or Wdr24A/A MEFs. Indicated cells were deprived of amino acids for 60 min and restimulated with amino acids for 10 min as indicated. Scale bar, 10 μm. e, The imaging data in (d) were quantified. n = 15, 14, 11, 13. P = 1.05E-07, 0.08, 1.72E-07. Data are the mean ± s.d., two-tailed t-test. NS, not significant. ***P < 0.001. f, A working model to show how glucose regulates mTORC1 kinase activity through AMPK-mediated WDR24, Raptor and TSC2 phosphorylation.
