Extended Data Fig. 2: Verification of cell cycle synchronization of wild-type HeLa cells.
From: Allosteric regulation of CAD modulates de novo pyrimidine synthesis during the cell cycle
a, western blots of canonical cell cycle indicating proteins, cyclin F and phosphorylated histone H3, and de novo pyrimidine proteins of interest, CAD and DHODH. b, Measurements of cell parameters after DTB release: cell number, mean cell volume, and total cell/culture volume in absolute or relative values. Data are the mean ± s.d. (n = 3, technical replicates, 5 independent experiments). c, EdU incorporation of cells synchronized by DTB. d, Volumetric distribution of cells synchronized by DTB.
