Extended Data Fig. 5: MT2 suppresses thermogenesis in BAT.
From: Thermogenic adipocyte-derived zinc promotes sympathetic innervation in male mice
a-b, TH-positive sympathetic innervation staining in BAT (a, n = 3 biological independent samples) and TH protein expression in heart of WT and MT2 AKO under 4 °C (n = 4 biological independent samples). Scale bar (1000 μm). c-g, UCP1 protein expression and HSL phosphorylation (c, n = 4 biological independent samples), H&E staining (Top) and UCP1 staining (Down) (d, n = 3 independent experiments) in BAT as well as body weight (e), Food intake (f) and activity (g) (n = 6 biological independent samples) of WT and MT2 AKO mice under 4 °C. Scale bar (50 μm). h, Thermogenic genes expression in beige adipocytes from WT and MT2 AKO mice (n = 4 biological independent samples). i-k, Body weight (i), food intake (j) and activity (k) of WT and MT2 AKO mice with surgical transection of sympathetic neuron fibers in scWAT and BAT (n = 6 biological independent samples). l-q, Body weight and food intake (l, m, n = 8 biological independent samples), UCP1 and TH immunoblot (Down) in transplanted cells (n, n = 3 biological independent samples), oxygen consumption (o, n = 8 biological independent samples), thermogenic genes expression in endogenous scWAT (p, n = 8 biological independent samples) and BAT (q, n = 8 biological independent samples) of C57BL6 mice transplanted with WT beige adipocytes (Trans-WT) and MT2-KO beige adipocytes (Trans-KO) under 4 °C. r, Mt2 mRNA levels in scWAT, BAT, Liver and Kidney from Ad-GFP and Ad-MT2 injected mice (n = 5 biological independent samples). s, Co-staining of adenovirus infected cells (GFP positive) with adipocytes (Perilipin positive) and sympathetic neurons (TH positive) (n = 3 independent experiments). Scale bar (50 μm). t, TH-positive sympathetic innervation staining in BAT of C57BL6 mice locally-injected with Ad-GFP and Ad-MT2 in scWAT and BAT under 4 °C (n = 3 biological independent samples). Scale bar (1000 μm). u-z, Thermogenic genes expression (u, n = 7 biological independent samples), UCP1 protein expression and HSL phosphorylation (v, n = 4 biological independent samples), H&E staining (Top) and UCP1 staining (Down) (w, n = 3 independent experiments) in BAT as well as body weight (x), food intake (y) and activity (z) (n = 7 biological independent samples) of C57BL6 mice locally-injected with Ad-GFP and Ad-MT2 under 4 °C. Scale bar (50 μm). Error bars represent ±SEM, *p < 0.05, **p < 0.01, ***p < 0.001. n.s., not significant. P values were determined by unpaired two-tailed Student’s t-test (a-c, g-k, n, p-z), two-way ANOVA (e-f, l-m) and two-sided analysis of covariance (ANCOVA) (o).
