Extended Data Fig. 2: Referred to Fig. 1.
From: Primary cilia sense glutamine availability and respond via asparagine synthetase
a, Quantification of cilia length in one representative experiment in mIMCD3 and MDCK after 24 hours culture in HBSS ± L-Glutamine (Q) (4 mM). Shared control (HBSS) with Fig. 1b. b, Left: analysis of OCR measurement in one representative experiment in MEF after 16 hours culture in either HBSS (blue) or HBSS + D-(+)-Glucose (Glc) (20 mM) (green) or Q (4 mM) (red) in basal condition and after sequential addition of O, FCCP, and A/R. Right: quantification of basal respiration, ATP-production coupled respiration, and maximal respiration as in Left. c, Left: analysis of OCR measurement in one representative experiment in MEF after 4 hours culture in HBSS (blue) followed by acute injection (+) of either Glc (green) or Q (red) and after sequential addition of O, FCCP and A/R. Right: quantification of basal respiration, acute response, ATP-production coupled respiration, and maximal respiration as in Left. d, Left: analysis of ECAR measurement referred to Fig. 1h. Right: analysis of ECAR measurement referred to b. Data in dot and bar plots are mean ± SD. Statistical analysis: Student’s unpaired two-tailed t-test or one-way ANOVA, followed by Tukey’s multiple comparisons test; ns: not significant, ****p < 0.0001. n reported in brackets.
