Extended Data Fig. 3: Referred to Fig. 1.
From: Primary cilia sense glutamine availability and respond via asparagine synthetase
a, Scheme of Mitochondrial Respiratory Chain Complexes targets of rotenone, antimycin, and oligomycin. b, Quantification of cilia length in one representative experiment in MEF and hRPE after 24 hours culture in DMEM or DMEM/F12 + 0% FBS ± either A/R or O. c, Western blot for pAMPKT172 of total cell lysates from MEF cultured for 1, 2, 4, 6 hours in HBSS or for 4 hours in either DMEM + 10% FBS ± AICAR (1 mM) or Metformin (2 mM). Vinculin is used as loading control. d, Representative IF images of one representative experiment after 24 hours culture in either DMEM/F12 + 10% FBS ± vehicle (veh) or AICAR. Cilia (ARL13B, green), nuclei (DAPI, blue). Scale bar: 5 µm. e, Left: Quantification of cilia length in one representative experiment in hRPE after 24 hours culture in the indicated conditions. Right: % of ciliated cells in one representative experiment in hRPE after 24 hours culture in the indicated conditions. f, Left: Quantification of cilia length in one representative experiment in mIMCD3 after 24 hours culture in the indicated conditions. Right: % of ciliated cells in one representative experiment in mIMCD3 after 24 hours culture in the indicated conditions. Data in dot and bar plots are mean ± SD. Statistical analysis: Student’s unpaired two-tailed t-test or one-way ANOVA, followed by Tukey’s multiple comparisons test; ns: not significant, ****p < 0.0001. n reported in brackets.
