Extended Data Fig. 4: Referred to Fig. 2.
From: Primary cilia sense glutamine availability and respond via asparagine synthetase
a, Left: experimental design of mice treatment: Crl (Fed), Fasted for 24 hours. Center: quantification of cilia length in one representative experiment in kidney sections of mice treated as in Left. Right: average from 3 independent experiments of glycaemia values in mice treated as in Left. b, Left: experimental design of mice treatment: Crl (Fed), Fasted for 48 hours. Center: quantification of cilia length in one representative experiment in kidney sections of mice treated as in Left. Right: average from 3 independent experiments of glycaemia values in mice treated as in Left. c, Left: experimental design of mice treatment: Crl (Fed), Fasted for 48 hours. Right: average from 3 independent experiments of Q concentration in the serum of mice treated as in Left. d, Scheme of renal tubular segment with Cre-recombinase driven inactivation of Opa1 gene. e, Left: Transmission Electron Microscopy (TEM) images of kidney sections of control (Crl) and Opa1 KO mice at P2. Scale bar: 1 µm. Dashed Dots indicate mitochondria. Right: Enzymatic activity staining for Succinate Dehydrogenase (SDH) and Cytochrome c Oxidase (COX) of kidney sections of Crl and Opa1 KO mice at P30 with zoom in. Dashed dots indicate medulla edge. Scale bar: 500 µm. f, Left: representative IF images of kidney sections of Crl and Opa1 KO mice at P30. Right: quantification of cilia length in one representative experiment of DBA + tubular cells in kidney sections of Crl and Opa1 KO mice at P30. Cilia (ARL13B, green), DBA + tubular cells (DBA, red) nuclei (DAPI, blue). Scale bar: 5 µm. Arrows indicate cilia in the insets. Data in dot and bar plots are mean ± SD. Statistical analysis: Student’s unpaired two-tailed t-test; ns: not significant, ****p < 0.0001. n reported in brackets.
