Extended Data Fig. 3: Characterization of Myf5Cre-Pcyt2 mice.
From: PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing
(A) Body weights of control and Myf5Cre-Pcyt2 male mice at P1 and P4. (B) Body length gains of control and Myf5Cre-Pcyt2 male mice. n = 6 per group for body length analysis. (C) Body weights of 2 months old control and Myf5Cre-Pcyt2 female mice. (D) Body lengths of 2 months old control and Myf5Cre-Pcyt2 female mice. (E) Skeletal muscle and tissue weight isolated from (E) 10 day old control (n = 6) and Myf5Cre-Pcyt2 (n = 8) and (F) 2 months old (P56) control (n = 8) and Myf5Cre-Pcyt2 (n = 7) littermate male mice. QA, quadriceps; GC, gastrocnemius; TA, tibialis anterior muscles. Liver and spleen weights are shown as controls. Scale bars 1 cm. (G, H) Gross skeletal muscle appearance of 56 days old control and Myf5Cre-Pcyt2 male littermates. (I) mRNA TPM levels of enzymes from the PE and PC branch of the phospholipid synthesis Kennedy pathway. n = 3 mice per group. Data are shown as means ± SEM. Data are shown as means ± SEM. Each dot represents data point from individual mice unless stated otherwise. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, n.s. not significant (unpaired Student t-test).
