Extended Data Fig. 6: Muscle inflammation and metabolic assessment of Myf5Cre-Pcyt2 mice.
From: PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing
(A) Grip strength of 6 months old control and Myf5Cre-Pcyt2 females. Each dot represents one mouse, values are average of three measurements per mouse. (B) Representative electron microscopy images of quadriceps of 15 months old control and Myf5Cre-Pcyt2 male mice. Note accumulation of tubular aggregates in the mutant animals (red arrows). Representative images of 3 animals per group are shown. Scale bar 2 µm. (C) Characterization of muscle inflammation in 12 months old Myf5Cre-Pcyt2 male mice. Helper T cells (CD4+) and cytotoxic T cells (CD8+) are shown. Scale bar 100 µm for H&E stained and 50 µm for immune cell staining. Representative staining of 3 animals per group are shown. (D) Inflammatory cytokine levels in the quadriceps of 12 months old Myf5Cre-Pcyt2 male mice. (E) Fed blood glucose levels on normal chow diet of 8 months old control and Myf5Cre-Pcyt2 male mice. (F) Food consumption analysis of 6 month and 8 months old control and Myf5Cre-Pcyt2 mice (G) Cage activity of 6 months old male control and Myf5Cre-Pcyt2 mice (n = 12 per group). Multiple ANOVA was used to analyse the data. (H) Energy expenditure of 6 months old male control and Myf5Cre-Pcyt2 mice during the resting (light) and active (dark) phases. (I) Cage activity under thermoneutrality of 6 months old male control and Myf5Cre-Pcyt2 mice (n = 6 per group). Multiple ANOVA was used to analyse the data. (J) Energy expenditure of 6 months old male control and Myf5Cre-Pcyt2 mice during the resting (light) and active (dark) phases under thermoneutrality. (K) Grip strength assessment of 6 months old male control and Myf5Cre-Pcyt2 mice under thermoneutrality. Data are shown as means ± SEM. Each dot represents data point from individual mice unless stated otherwise. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, n.s. not significant. Unpaired Student t-test with Welch correction was used for statistical analysis unless stated otherwise.
