Extended Data Fig. 9: Assessment of mitochondrial homeostasis and SS-31 treatment.
From: PCYT2-regulated lipid biosynthesis is critical to muscle health and ageing
(A, B) Muscle mitochondrial function assessed by measurements of complex II linked activity on isolated mitochondria from 2 months and from (C) 6 months old control and Myf5Cre-Pcyt2 male mice respectively. Paired Student t-test was used for statistical analysis. (D, E) Ultrastructure and total numbers of muscle mitochondria from 8 months -old control (n = 6) and Myf5Cre-Pcyt2 male mice (n = 6). Scale bar 200 nm. Unpaired Student t-test with Welch correction was used for statistical analysis (F) Function of muscle mitochondria under increasing concentrations of phosphoethanolamine, as assessed by measurements of complex I linked activity on isolated mitochondria from 2 months old control and Myf5Cre-Pcyt2 male mice. N = 3 mice per group. Two-Way ANOVA with multiple comparison followed by Bonferroni correction was used for statistical analysis. (G, H) Grip strength and organ weight measurements of 6 months old control (vehicle) and Myf5Cre-Pcyt2 male mice that have been treated with either vehicle or ss-31 compound for two months. Credit: IMP-IMBA Graphics Department. Data are shown as means ± SEM. Multiple comparison One-Way ANOVA with Dunnett correction was used for statistical analysis.
