Extended Data Fig. 4: H2O2 levels associated with cell attachment.
From: The GAPDH redox switch safeguards reductive capacity and enables survival of stressed tumour cells
(a) The roGFP2-Orp1 405 nm/488 nm fluorescence ratio as measured by flow cytometry in WT and mutant (Y314F) HAP1 cells grown in adherent monolayer culture, following treatment with either 20 mM diamide (Dia) (full oxidation) or 100 mM dithiothreitol (DTT) (full reduction). Left panel: representative histogram. Right panel: Data are presented as mean values ± SD, based n = 3 biological replicates (unpaired two-tailed t test). ns: non-significant, based on a two-tailed unpaired t-test.
