Extended Data Fig. 6: FACS Gating Strategies.
From: The GAPDH redox switch safeguards reductive capacity and enables survival of stressed tumour cells
(a) Gating scheme for flow cytometry-based analysis of ROS levels in erythrocytes from peripheral blood (Fig. 7a). Sequential flow cytometry gates were used to define: CD41- Ter119+ erythroid cells; CD71-, Ter119+ erythrocytes. The level of fluorescence of the DCFDA stain was assessed for this cell population within the FITC channel of the flow cytometer. (b) Gating scheme for flow cytometry-based identification of MEP cells from bone marrow (Fig. 7b). Sequential flow cytometry gates were used to define: live leukocytes based on forward scatter versus side scatter; single cells based on forward scatter area versus forward scatter height; lineage negative cells; c-Kit+, Sca-1- cells; CD34-, CD16/32- MEPs (indicated by red box).
