Fig. 1: Dysregulated lipid metabolism in Irg1−/− macrophages.
a, BMMs from four wild-type and four Irg1−/− mice were analyzed for broad metabolomics by mass spectrometry. For each metabolite, a ratio was computed by dividing the metabolite level by the relative mean for all eight samples (four wild-type and four Irg1−/−). The heat map depicts log2-transformed ratios for metabolites significantly different; all metabolites shown are *P < 0.05 between genotypes, as determined by two-sided multiple t-tests (one per row). b, Itaconate production was confirmed in cultured BMMs by culturing cells in the presence of 50 μg ml−1 macrophage colony-stimulating factor. At the indicated times, supernatant was collected and analyzed for itaconate using mass spectrometry. The graph depicts results from triplicate bone-marrow cultures. c, Peritoneal resident macrophages were isolated from the indicated mice. Lipid staining was visualized using flow cytometric analysis of BODIPY 493/503 staining. The graph illustrates results from ten mice. Data are presented as mean ± s.e.m. A two-sided analysis of variance (ANOVA) was performed between each group and the wild-type control (**P = 0.0015; ***P = 0.0001). As a positive control, wild-type mice were treated with 50 μg LPS 24 h before the isolation of peritoneal macrophages.
