Fig. 3: Exacerbated fatty liver disease in Irg1−/− mice after WD diet feeding.
a, ORO staining of frozen liver tissue sections. Each image is representative of ten non-overlapping fields from five mice per group and repeated in two separate experiments; scale bar, 300 μm). b, The sum areas of ORO staining were quantified using ImageJ software (*P = 0.02 for the indicated comparisons; #P = 0.01 compared to the wild-type/control diet sample; n = 10). c, Representative H&E staining of liver tissues. The image depicted is representative of five non-overlapping fields from three mice. Scale bar, 300 μM; ×10 magnification. d, Liver weights of five mice per group (*P = 0.036; **P = 0.009). e, Mesenteric fat weights (five mice per group; **P = 0.009; ***P = 0.0006; NS, not significant). f, Serum levels of free fatty acids (n = 5 per group; *P = 0.043; ***P = 0.0006; NS, not significant). g, Liver triglycerides (n = 4 per group; *P = 0.013; **P = 0.001; NS, not significant). Data are presented as mean ± s.e.m. A two-sided ANOVA with multiple comparisons was used for statistical analyses.
