Fig. 6: Loss of hepatic and adipose macrophages in global and myeloid-deficient Irg1 mice fed WD.
a, Macrophage (F4/80) staining of formalin-fixed liver tissues, visualized using DAB chromogen. Each image is representative of ten non-overlapping fields from five mice per group and repeated in two separate experiments; scale bar, 300 μm). b, The F4/80-stained areas of 14 images per group were quantitated using ImageJ software (*P = 0.015; ***P = 0.0002). c, Reduction of hepatic crown-like structures (CLSs); F4/80+ macrophages forming a ring-like structure. The number of CLSs were manually counted on 14 images per group (****P < 0.0001). d, Macrophage (F4/80) staining of formalin-fixed adipose tissue, visualized using alkaline phosphatase chromogen (scale bar, 500 μM). Each image is representative of five non-overlapping fields from five mice per group. e, Quantitation of CLSs in adipose tissue (n = 25 images; **P = 0.002). CLSs in adipose tissue surround dying adipocytes in a crown-like pattern. Data are presented as mean ± s.e.m. A two-sided ANOVA with multiple comparisons was used for statistical analyses.
