Fig. 2: Metabolome differences between small and large intestine of male SPF mice.
a, Differential analysis of metabolite concentrations between small and large intestinal samples in lumen (upper) and mucus (lower). Data points represent mean fold change values calculated between ten small intestinal and four large intestinal sites, for five male mice. Negative and positive values represent higher concentrations in the small or large intestine, respectively. The y axis displays −log10-transformed P values, calculated using a two-sided paired-sample Student’s t-test with Benjamini–Hochberg correction for multiple testing. Metabolites with significantly different concentrations (absolute log2(fold change) ≥ 2, corrected P ≤ 0.05) are colour-coded according to the MetaCyc classification, as defined in the box (bottom left). b, Significantly changing metabolites between the small and large intestine in the lumen and mucus from differential analysis in Fig. 1a (absolute log2(fold change) ≥ 2, corrected P ≤ 0.05). P values were calculated using a two-sided paired-sample Student’s t-test with Benjamini–Hochberg correction for multiple testing. Dot colours denote fold change and dot size denotes significance. Metabolites are classified according to MetaCyc, as defined in the box (bottom left). c,d, Spatial profiles of histidine and tryptophan (c) and glycerate and 4-hydroxyphenylacetate (d) over 15 intestinal sites in SPF mucus or lumen, respectively. Lines with shaded areas indicate the moving average of the mean ± s.e.m. of concentration measurements from five male mice. Abbreviations: cec, caecum; col, colon; duo, duodenum; ile, ileum; jej, jejunum; sto, stomach; TMAO, trimethylamine N-oxide.
