Extended Data Fig. 3: Hierarchical clustering of male SPF large intestinal samples.
a, b – Hierarchical clustering analysis of metabolite abundances from four large intestinal luminal content (a) and mucus (b) samples in male SPF mice. Abundances of 128 metabolites are shown as z-scores of normalized concentrations averaged from five male mice per sampling site in the colon (cecum, ascending, transverse and descending colon). Hierarchical clustering was performed only on these four values. Significantly changing metabolites are marked with boxes and asterisks (p-value ≤ 0.05). P-values were calculated using a two-sided paired-sample Student’s t-test. Color key of the heatmap indicates low to high relative abundance of metabolites throughout the large intestine. c – Distribution of peak concentrations in luminal content (top) and mucus (bottom) large intestine, with respect to the four regions is depicted. Four bars denote the regions, y-axis shows frequency of peak metabolite concentrations occurring in the respective region. Abbreviations: cec – cecum, acol – ascending colon, tcol – transverse colon, and dcol – descending colon.
